| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Li, Chelsea T (8)
|
VACVAC |
42.91 |
44.48 |
1.57 |
| 2 |
Mannato, Bryn K (8)
|
VATIDE |
|
47.60 |
- |
| 3 |
Stowell, Olivia R (7)
|
VATIDE |
|
48.14 |
- |
| 4 |
Bradshaw, Piper L (8)
|
VATIDE |
|
53.45 |
- |
| 5 |
Raeburn, Morgan Delaney (8)
|
VACVAC |
|
54.24 |
- |
| 6 |
Nicosia, Charlotte R (8)
|
VATIDE |
|
54.28 |
- |
| 7 |
Boyer, Abigail E (8)
|
VATIDE |
|
55.03 |
- |
| 8 |
Hamilton, Gracie A (8)
|
VATIDE |
|
1:00.98 |
- |
| 9 |
Neville, Kailyn R (8)
|
VATIDE |
|
1:13.49 |
- |
| 10 |
Karunaratne, Olivia C (7)
|
VATIDE |
|
1:16.79 |
- |
|
Culley, Guilianna A (8)
|
VATIDE |
|
DQ |
- |
|
Hathorn, Margaret G (6)
|
VATIDE |
|
DQ |
- |
|
Klimkiewicz, Emma R (7)
|
VATIDE |
|
DQ |
- |
|
Slye, Emily S (7)
|
VATIDE |
|
DQ |
- |